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Article

Reduced PCR-generated errors from a hybrid capture-based NGS assay for HLA typing.

Human immunology - 1 Apr 2021

Brown Nicholas K, Merkens Hanneke, Rozemuller Erik H, Bell Derrick, Bui Thanh-Mai, Kearns Jane

Abstract excerpt

Next generation sequencing (NGS) assays are state of the art for HLA genotyping. To sequence on an Illumina sequencer, the DNA of interest must be enriched, fragmented, and bookended with known oligonucleotide sequences, a process known as library construction. Many HLA genotyping assays enrich the target loci by long-range PCR (LR-PCR), prior to fragmentation. This PCR step has been reported to introduce errors...

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PMID: 33676750
DOI: 10.1016/j.humimm.2021.02.010

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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