A novel single cDNA amplicon pyrosequencing method for high-throughput, cost-effective sequence-based HLA class I genotyping.

doi:10.1016/j.humimm.2010.07.012

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Article

A novel single cDNA amplicon pyrosequencing method for high-throughput, cost-effective sequence-based HLA class I genotyping.

Human immunology - 1 Oct 2010

Lank Simon M, Wiseman Roger W, Dudley Dawn M, O'Connor David H

Abstract excerpt

Human leukocyte antigen (HLA) genotype influences the immune response to pathogens and transplanted tissues; accurate HLA genotyping is critical for clinical and research applications. Sequence-based HLA typing is limited by the cost of Sanger sequencing genomic DNA (gDNA) and resolving cis/trans ambiguities, hindering both studies correlating high-resolution genotype with clinical outcomes, and...

Topics

Alleles
Cost-Benefit Analysis
DNA Primers
DNA, Complementary
Genes, MHC Class I
High-Throughput Nucleotide Sequencing
Histocompatibility Testing
Humans
Nucleic Acid Amplification Techniques
Sequence Analysis, DNA

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Identifiers

PMID: 20650293
DOI: 10.1016/j.humimm.2010.07.012
PMCID: PMC3191540

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