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Article

Next-generation HLA sequencing using the 454 GS FLX system.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2013

Trachtenberg Elizabeth A, Holcomb Cherie L

Abstract excerpt

Next-generation sequencing (NGS) of HLA class I and II loci (HLA-A, HLA-B, HLA-C, DRB1, DRB3, DRB4, DRB5, DQA1, DQB1, DPB1) is described here in detail using the 454 Life Sciences GS FLX System and Titanium chemistry. An overview of the protocol with our experience on sequence performance efficiencies, read depth and ambiguity analyses using the GS FLX System are also presented. A total of 14 HLA primer pairs...

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Identifiers

PMID: 23775738
DOI: 10.1007/978-1-62703-493-7_10
PMCID: PMC4196724

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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