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Article

Next-generation HLA sequencing using the 454 GS FLX system.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2013

Trachtenberg Elizabeth A, Holcomb Cherie L

Abstract excerpt

Next-generation sequencing (NGS) of HLA class I and II loci (HLA-A, HLA-B, HLA-C, DRB1, DRB3, DRB4, DRB5, DQA1, DQB1, DPB1) is described here in detail using the 454 Life Sciences GS FLX System and Titanium chemistry. An overview of the protocol with our experience on sequence performance efficie...

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Identifiers

PMID: 23775738
DOI: 10.1007/978-1-62703-493-7_10
PMCID: PMC4196724

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026. Prepared record publication-23775738-2026-09-04.2. Manifest publication-graph-cohort-2026-09-04.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 4 Sept 2026.

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