Article
CRISPR-Cas9 genome editing of a single regulatory element nearly abolishes target gene expression in mice--brief report.
Arteriosclerosis, thrombosis, and vascular biology - 1 Feb 2015
Han Yu, Slivano Orazio J, Christie Christine K, Cheng Albert W, Miano Joseph M
Abstract excerpt
OBJECTIVE: To ascertain the importance of a single regulatory element in the control of Cnn1 expression using CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9) genome editing. APPROACH AND RESULTS: The CRISPR/Cas9 system was used to produce 3 of 18 founder mice carrying point mutations in an intronic CArG box of the smooth muscle cell-restricted Cnn1 gene. Each...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.