Article
Variation in zygotic CRISPR/Cas9 gene editing outcomes generates novel reporter and deletion alleles at the Gdf11 locus.
Scientific reports - 9 Dec 2019
Goldstein Jill M, Valido Austin, Lewandowski Jordan P, Walker Ryan G, Mills Melanie J, Messemer Kathleen A, Besseling Paul, Lee Kyu Ha, Wattrus Samuel J, Cho Miook, Lee Richard T, Wagers Amy J
Abstract excerpt
Recent advances in CRISPR/Cas gene editing technology have significantly expanded the possibilities and accelerated the pace of creating genetically engineered animal models. However, CRISPR/Cas-based strategies designed to precisely edit the genome can often yield unintended outcomes. Here, we report the use of zygotic CRISPR/Cas9 injections to generate a knock-in GFP reporter mouse at the Gdf11 locus....
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.