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Conditional knockouts generated by engineered CRISPR-Cas9 endonuclease reveal the roles of coronin in C. elegans neural development.

Developmental cell - 8 Sept 2014

Shen Zhongfu, Zhang Xianliang, Chai Yongping, Zhu Zhiwen, Yi Peishan, Feng Guoxin, Li Wei, Ou Guangshuo

Abstract excerpt

Conditional gene knockout animals are valuable tools for studying the mechanisms underlying cell and developmental biology. We developed a conditional knockout strategy by spatiotemporally manipulating the expression of an RNA-guided DNA endonuclease, CRISPR-Cas9, in Caenorhabditis elegans somatic cell lineages. We showed that this somatic CRISPR-Cas9 technology provides a quick and efficient approach to generate...

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PMID: 25155554
DOI: 10.1016/j.devcel.2014.07.017

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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