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Article

Enhanced specificity and efficiency of the CRISPR/Cas9 system with optimized sgRNA parameters in Drosophila.

Cell reports - 6 Nov 2014

Ren Xingjie, Yang Zhihao, Xu Jiang, Sun Jin, Mao Decai, Hu Yanhui, Yang Su-Juan, Qiao Huan-Huan, Wang Xia, Hu Qun, Deng Patricia, Liu Lu-Ping, Ji Jun-Yuan, Li Jin Billy, Ni Jian-Quan

Abstract excerpt

The CRISPR/Cas9 system has recently emerged as a powerful tool for functional genomic studies in Drosophila melanogaster. However, single-guide RNA (sgRNA) parameters affecting the specificity and efficiency of the system in flies are still not clear. Here, we found that off-target effects did no...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 25437567
DOI: 10.1016/j.celrep.2014.09.044
PMCID: PMC4250831

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026. Prepared record publication-25437567-2026-09-04.2. Manifest publication-graph-cohort-2026-09-04.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 4 Sept 2026.

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