Article
Enhanced specificity and efficiency of the CRISPR/Cas9 system with optimized sgRNA parameters in Drosophila.
Cell reports - 6 Nov 2014
Ren Xingjie, Yang Zhihao, Xu Jiang, Sun Jin, Mao Decai, Hu Yanhui, Yang Su-Juan, Qiao Huan-Huan, Wang Xia, Hu Qun, Deng Patricia, Liu Lu-Ping, Ji Jun-Yuan, Li Jin Billy, Ni Jian-Quan
Abstract excerpt
The CRISPR/Cas9 system has recently emerged as a powerful tool for functional genomic studies in Drosophila melanogaster. However, single-guide RNA (sgRNA) parameters affecting the specificity and efficiency of the system in flies are still not clear. Here, we found that off-target effects did not occur in regions of genomic DNA with three or more nucleotide mismatches to sgRNAs. Importantly, we document for a...
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