Article
CRISPRscan: designing highly efficient sgRNAs for CRISPR-Cas9 targeting in vivo.
Nature methods - 1 Oct 2015
Moreno-Mateos Miguel A, Vejnar Charles E, Beaudoin Jean-Denis, Fernandez Juan P, Mis Emily K, Khokha Mustafa K, Giraldez Antonio J
Abstract excerpt
CRISPR-Cas9 technology provides a powerful system for genome engineering. However, variable activity across different single guide RNAs (sgRNAs) remains a significant limitation. We analyzed the molecular features that influence sgRNA stability, activity and loading into Cas9 in vivo. We observed that guanine enrichment and adenine depletion increased sgRNA stability and activity, whereas differential sgRNA...
Topics
- 3' Untranslated Regions
- Adenine
- Algorithms
- Animals
- CRISPR-Cas Systems
- Clustered Regularly Interspaced Short Palindromic Repeats
- Embryo, Nonmammalian
- Female
- Gene Expression Regulation
- Gene Targeting