CRISPR-Cas9-mediated pinpoint microbial genome editing aided by target-mismatched sgRNAs.

Abstract excerpt

Genome editing has been revolutionized by the CRISPR-Cas9 system. CRISPR-Cas9 is composed of single-molecular guide RNA (sgRNA) and a proteinaceous Cas9 nuclease, which recognizes a specific target sequence and a protospacer adjacent motif (PAM) sequence and, subsequently, cleaves the targeted DNA sequence. This CRISPR-Cas9 system has been used as an efficient negative-selection tool to cleave unedited or...

Topics

• Base Pair Mismatch
• CRISPR-Associated Protein 9
• CRISPR-Cas Systems
• Escherichia coli
• Gene Editing
• Genome, Bacterial
• Mutagenesis
• Mutation
• Plasmids
• RNA