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Article

Exploiting post-transcriptional regulation to probe RNA structures in vivo via fluorescence.

Nucleic acids research - 1 Jan 2015

Sowa Steven W, Vazquez-Anderson Jorge, Clark Chelsea A, De La Peña Ricardo, Dunn Kaitlin, Fung Emily K, Khoury Mark J, Contreras Lydia M

Abstract excerpt

While RNA structures have been extensively characterized in vitro, very few techniques exist to probe RNA structures inside cells. Here, we have exploited mechanisms of post-transcriptional regulation to synthesize fluorescence-based probes that assay RNA structures in vivo. Our probing system involves the co-expression of two constructs: (i) a target RNA and (ii) a reporter containing a probe complementary to a...

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Identifiers

PMID: 25416800
DOI: 10.1093/nar/gku1191
PMCID: PMC4333371

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Source receipt: pubmed, retrieved 7 Sept 2026.

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