Defining the chemical groups essential for Tetrahymena group I intron function by nucleotide analog interference mapping.

doi:10.1073/pnas.94.7.2903

Back to search

Article

Defining the chemical groups essential for Tetrahymena group I intron function by nucleotide analog interference mapping.

Proceedings of the National Academy of Sciences of the United States of America - 1 Apr 1997

Strobel S A, Shetty K

Abstract excerpt

Improved atomic resolution biochemical methods are needed to identify the chemical groups within an RNA that are essential to its activity. As a step toward this goal, we report the use of 5'-O-(1-thio)inosine monophosphate (IMP alphaS) in a nucleotide analog interference mapping (NAIM) assay tha...

Topics

Animals
Exons
Guanosine
Inosine
Introns
Mutagenesis, Site-Directed
Mutation
Tetrahymena
Thionucleotides

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.