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Article

Single-molecule imaging of transcriptionally coupled and uncoupled splicing.

Cell - 23 Nov 2011

Vargas Diana Y, Shah Khyati, Batish Mona, Levandoski Michael, Sinha Sourav, Marras Salvatore A E, Schedl Paul, Tyagi Sanjay

Abstract excerpt

Introns are removed from pre-mRNAs during transcription while the pre-mRNA is still tethered to the gene locus via RNA polymerase. However, during alternative splicing, it is important that splicing be deferred until all of the exons and introns involved in the choice have been synthesized. We have developed an in situ RNA imaging method with single-molecule sensitivity to define the intracellular sites of...

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Identifiers

PMID: 22118462
DOI: 10.1016/j.cell.2011.10.024
PMCID: PMC3245879

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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