Article
A general method for rapid and nondenaturing purification of RNAs.
RNA (New York, N.Y.) - 1 Jun 2004
Kieft Jeffrey S, Batey Robert T
Abstract excerpt
A key bottleneck in RNA structural studies is preparing milligram quantities of RNA, and current techniques have changed little in over a decade. To address this, we have developed an affinity tag-based purification method of RNA oligonucleotides. The tag is attached to the 3'-end of almost any desired RNA sequence, allowing for the rapid and specific removal of the RNA of interest directly from in vitro...
Topics
- Affinity Labels
- Animals
- Base Sequence
- Crystallization
- Crystallography, X-Ray
- Introns
- Magnetic Resonance Spectroscopy
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- RNA
- RNA, Protozoan