Article
High-throughput mutate-map-rescue evaluates SHAPE-directed RNA structure and uncovers excited states.
RNA (New York, N.Y.) - 1 Nov 2014
Tian Siqi, Cordero Pablo, Kladwang Wipapat, Das Rhiju
Abstract excerpt
The three-dimensional conformations of noncoding RNAs underpin their biochemical functions but have largely eluded experimental characterization. Here, we report that integrating a classic mutation/rescue strategy with high-throughput chemical mapping enables rapid RNA structure inference with unusually strong validation. We revisit a 16S rRNA domain for which SHAPE (selective 2'-hydroxyl acylation with primer...
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