Article
A two-dimensional mutate-and-map strategy for non-coding RNA structure.
Nature chemistry - 30 Oct 2011
Kladwang Wipapat, VanLang Christopher C, Cordero Pablo, Das Rhiju
Abstract excerpt
Non-coding RNAs fold into precise base-pairing patterns to carry out critical roles in genetic regulation and protein synthesis, but determining RNA structure remains difficult. Here, we show that coupling systematic mutagenesis with high-throughput chemical mapping enables accurate base-pair inference of domains from ribosomal RNA, ribozymes and riboswitches. For a six-RNA benchmark that has challenged previous...
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