Article
Selective 2'-hydroxyl acylation analyzed by primer extension and mutational profiling (SHAPE-MaP) for direct, versatile and accurate RNA structure analysis.
Nature protocols - 1 Nov 2015
Smola Matthew J, Rice Greggory M, Busan Steven, Siegfried Nathan A, Weeks Kevin M
Abstract excerpt
Selective 2'-hydroxyl acylation analyzed by primer extension (SHAPE) chemistries exploit small electrophilic reagents that react with 2'-hydroxyl groups to interrogate RNA structure at single-nucleotide resolution. Mutational profiling (MaP) identifies modified residues by using reverse transcriptase to misread a SHAPE-modified nucleotide and then counting the resulting mutations by massively parallel sequencing....
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.