Article
Template switching enables chemical probing of native RNA structures.
RNA (New York, N.Y.) - 16 Dec 2024
Hall Ian, O'Steen Martin, Gold Sophie, C Keane Sarah, Weidmann Chase A
Abstract excerpt
RNAs are often studied in nonnative sequence contexts to facilitate structural studies. However, seemingly innocuous changes to an RNA sequence may perturb the native structure and generate inaccurate or ambiguous structural models. To facilitate the investigation of native RNA secondary structure by selective 2' hydroxyl acylation analyzed by primer extension (SHAPE), we engineered an approach that couples...
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