Article
A mutate-and-map strategy accurately infers the base pairs of a 35-nucleotide model RNA.
RNA (New York, N.Y.) - 1 Mar 2011
Kladwang Wipapat, Cordero Pablo, Das Rhiju
Abstract excerpt
We present a rapid experimental strategy for inferring base pairs in structured RNAs via an information-rich extension of classic chemical mapping approaches. The mutate-and-map method, previously applied to a DNA/RNA helix, systematically searches for single mutations that enhance the chemical accessibility of base-pairing partners distant in sequence. To test this strategy for structured RNAs, we have carried...
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