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Article

Quantitative analysis of mammalian translation initiation sites by FACS-seq.

Molecular systems biology - 28 Aug 2014

Noderer William L, Flockhart Ross J, Bhaduri Aparna, Diaz de Arce Alexander J, Zhang Jiajing, Khavari Paul A, Wang Clifford L

Abstract excerpt

An approach combining fluorescence-activated cell sorting and high-throughput DNA sequencing (FACS-seq) was employed to determine the efficiency of start codon recognition for all possible translation initiation sites (TIS) utilizing AUG start codons. Using FACS-seq, we measured translation from a genetic reporter library representing all 65,536 possible TIS sequences spanning the -6 to +5 positions. We found...

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Identifiers

PMID: 25170020
DOI: 10.15252/msb.20145136
PMCID: PMC4299517

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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