Article
Genomic phenotyping by barcode sequencing broadly distinguishes between alkylating agents, oxidizing agents, and non-genotoxic agents, and reveals a role for aromatic amino acids in cellular recovery after quinone exposure.
PloS one - 1 Jan 2013
Svensson J Peter, Quirós Pesudo Laia, McRee Siobhan K, Adeleye Yeyejide, Carmichael Paul, Samson Leona D
Abstract excerpt
Toxicity screening of compounds provides a means to identify compounds harmful for human health and the environment. Here, we further develop the technique of genomic phenotyping to improve throughput while maintaining specificity. We exposed cells to eight different compounds that rely on different modes of action: four genotoxic alkylating (methyl methanesulfonate (MMS), N-Methyl-N-nitrosourea (MNU),...
Topics
- Alkylating Agents
- Amino Acids, Aromatic
- Biosynthetic Pathways
- Carbamates
- Cell Cycle
- DNA Damage
- DNA Repair
- DNA, Fungal
- Dimethyl Sulfoxide
- Genes, Fungal
- Genomics
- Microbial Sensitivity Tests
- Mutation