Article
Barcode sequencing for understanding drug-gene interactions.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2012
Smith Andrew M, Durbic Tanja, Kittanakom Saranya, Giaever Guri, Nislow Corey
Abstract excerpt
With the advent of next-generation sequencing (NGS) technology, methods previously developed for microarrays have been adapted for use by NGS. Here we describe in detail a protocol for Barcode analysis by sequencing (Bar-seq) to assess pooled competitive growth of individually barcoded yeast deletion mutants. This protocol has been optimized on two sequencing platforms: Illumina's Genome Analyzer IIx/HiSeq2000...
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