Thermodynamic dissection of large-scale domain motions coupled with ligand binding of enzyme I.

Abstract excerpt

Domain motions are central to the biological functions of many proteins. The energetics of the motions, however, is often difficult to characterize when motions are coupled with the ligand binding. Here, we determined the thermodynamic parameters of individual domain motions and ligand binding of enzyme I (EI) using strategic domain-deletion mutants that selectively removed particular motions. Upon ligand...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Calorimetry
• Share to Ligands
• Share to Motion