Article
Double Mutant Cycles as a Tool to Address Folding, Binding, and Allostery.
International journal of molecular sciences - 15 Jan 2021
Pagano Livia, Toto Angelo, Malagrinò Francesca, Visconti Lorenzo, Jemth Per, Gianni Stefano
Abstract excerpt
Quantitative measurement of intramolecular and intermolecular interactions in protein structure is an elusive task, not easy to address experimentally. The phenomenon denoted 'energetic coupling' describes short- and long-range interactions between two residues in a protein system. A powerful method to identify and quantitatively characterize long-range interactions and allosteric networks in proteins or...
Topics
- Allosteric Regulation
- Allosteric Site
- Animals
- Biophysical Phenomena
- Computer Simulation
- Escherichia coli
- Humans
- Ligands
- Mass Spectrometry
- Mice
- Models, Molecular
- Molecular Conformation
- Mutagenesis, Site-Directed
- Mutation