A Simplified Method for Gene Knockout and Direct Screening of Recombinant Clones for Application in Paenibacillus polymyxa.

doi:10.1371/journal.pone.0068092

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Article

A Simplified Method for Gene Knockout and Direct Screening of Recombinant Clones for Application in Paenibacillus polymyxa.

PloS one - 1 Jan 2013

Kim Seong-Bin, Timmusk Salme

Abstract excerpt

BACKGROUND: Paenibacillus polymyxa is a bacterium widely used in agriculture, industry, and environmental remediation because it has multiple functions including nitrogen fixation and produces various biologically active compounds. Among these compounds are the antibiotics polymyxins, and the bacterium is currently being reassessed for medical application. However, a lack of genetic tools for manipulation of P....

Topics

Bacillus subtilis
Bacterial Proteins
Cloning, Molecular
Gene Knockout Techniques
Genetic Vectors
Homologous Recombination
Mutation
Paenibacillus polymyxa
Plasmids
Recombinant Proteins
Transformation, Genetic
alpha-Amylases
beta-Amylase

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Identifiers

PMID: 23826364
DOI: 10.1371/journal.pone.0068092
PMCID: PMC3694910

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