Marker-exchange mutagenesis and complementation strategies for the Gram-negative bacteria Xanthomonas oryzae pv. oryzae.

Abstract excerpt

This chapter describes methods for targeted knockouts using marker exchange mutagenesis and complementation of the Gram-negative bacteria Xanthomonas oryzae pv. oryzae. We have used these methods to demonstrate that type I secretion and modification systems are involved in avrXa21 activity of X. oryzae pv. oryzae.

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