Article

A method for increasing electroporation competency of Gram-negative clinical isolates by Polymyxin B nonapeptide

2022-01-12

Abstract excerpt

The study of clinically relevant bacterial pathogens relies on molecular and genetic approaches. However, the generally low transformation frequency among natural isolates poses technical hurdles to widely applying common methods in molecular biology, including transformation of large constructs, chromosomal genetic manipulation, and dense mutant library construction. Here we demonstrate that culturing clinical is...

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Literature Corpus work: 9ad1fd0c-e46e-5a37-8aa7-d448a3f18aa0
DOI: 10.21203/rs.3.rs-1248341/v1

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