Article
An efficient system for the generation of marked genetic mutants in members of the genus Burkholderia.
Plasmid - 1 Jan 2017
Shastri Sravanthi, Spiewak Helena L, Sofoluwe Aderonke, Eidsvaag Vigdis A, Asghar Atif H, Pereira Tyrone, Bull Edward H, Butt Aaron T, Thomas Mark S
Abstract excerpt
To elucidate the function of a gene in bacteria it is vital that targeted gene inactivation (allelic replacement) can be achieved. Allelic replacement is often carried out by disruption of the gene of interest by insertion of an antibiotic-resistance marker followed by subsequent transfer of the mutant allele to the genome of the host organism in place of the wild-type gene. However, due to their intrinsic...
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