Article
Versatile Vectors for Efficient Mutagenesis of Bradyrhizobium diazoefficiens and Other Alphaproteobacteria.
Applied and environmental microbiology - 1 May 2016
Ledermann Raphael, Strebel Silvan, Kampik Clara, Fischer Hans-Martin
Abstract excerpt
UNLABELLED: Analysis of bacterial gene function commonly relies on gene disruption or replacement followed by phenotypic characterization of the resulting mutant strains. Deletion or replacement of targeted regions is commonly achieved via two homologous recombination (HR) events between the bacterial genome and a nonreplicating plasmid carrying DNA fragments flanking the region to be deleted. The...
Topics
- Alphaproteobacteria
- Anti-Bacterial Agents
- Base Sequence
- Bradyrhizobium
- Chromosome Mapping
- Drug Resistance, Bacterial
- Gene Deletion
- Genetic Vectors
- Genetics, Microbial
- Genome, Bacterial
- Mutagenesis, Site-Directed
- Phenotype
- Plasmids
- Sequence Analysis, DNA
- Symbiosis