Article
A strategy to construct vector-free amylolytic strains through nondisruptive homologous recombination: application to Enterococcus faecalis.
Gene - 1 Sept 1992
Hols P, de Halleux S, Delcour J
Abstract excerpt
In order to direct chromosomal integration of the alpha-amylase-encoding gene from Bacillus licheniformis (amyL) under the control of expression and secretion signals from Enterococcus faecalis, the chromosomal fragment (named AB) from the pGIP3124 plasmid [Hols et al., Gene 118 (1992) 21-30] was...
Topics
- Amino Acid Sequence
- Bacillus
- Base Sequence
- Enterococcus faecalis
- Genetic Vectors
- Molecular Sequence Data
- Mutagenesis, Insertional
- Phenotype
- Plasmids
- Recombinant Fusion Proteins
- Recombination, Genetic
- Restriction Mapping
- Starch
- Transformation, Genetic
- alpha-Amylases