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Article

An allelic exchange system for compliant genetic manipulation of the select agents Burkholderia pseudomallei and Burkholderia mallei.

Gene - 1 Feb 2009

Hamad Mohamad A, Zajdowicz Sheryl L, Holmes Randall K, Voskuil Martin I

Abstract excerpt

Burkholderia pseudomallei and B. mallei are Gram-negative bacterial pathogens that cause melioidosis in humans and glanders in horses, respectively. Both bacteria are classified as category B select agents in the United States. Due to strict select-agent regulations, the number of antibiotic selection markers approved for use in these bacteria is greatly limited. Approved markers for B. pseudomallei include genes...

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Identifiers

PMID: 19010402
DOI: 10.1016/j.gene.2008.10.011
PMCID: PMC2646673

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