Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

In vitro phenotyping of recombinant hepatitis B virus containing the polymerase/reverse transcriptase gene from clinical isolates.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2013

Liu Yang, Kitrinos Kathryn M

Abstract excerpt

This chapter describes the procedures for the generation of recombinant hepatitis B virus (HBV) that contains the polymerase/reverse transcriptase (pol/RT) gene from clinical isolates. It also contains procedures for phenotypic testing of the recombinant HBV in HepG2 cells for susceptibility to nucleos(t)ide analogs. Amplification and cloning of the pol/RT gene as opposed to the full-length HBV genome from a...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Cell Culture Techniques
Share to DNA Replication

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Genes, pol

Share to Genetic Vectors

Share to Hep G2 Cells

Share to Hepatitis B virus

Share to Multiplex Polymerase Chain Reaction

Share to RNA-Directed DNA Polymerase

Source

Identifiers

PMID: 23821268
DOI: 10.1007/978-1-62703-484-5_14

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

In vitro phenotyping of recombinant hepatitis B virus containing the polymerase/reverse transcriptase gene from clinical isolates.PMID 23821268Read ↗

[Analysis and recombinant construction of HBV the reverse transcriptase gene with drug-resistant mutations from 40 patients with chronic hepatitis B].Semantic proximity Read ↗

Molecular diagnosis and treatment of drug-resistant hepatitis B virus.Semantic proximity Read ↗

HBV DNA replication mediated by cloned patient HBV reverse transcriptase genes from HBV genotypes A–H and its use in antiviral phenotyping assaysSemantic proximity Read ↗

Phenotypic assay of a hepatitis B virus strain carrying an rtS246T variant using a new strategy.Semantic proximity Read ↗

In vitro replication competence of a hepatitis B genotype D/A recombinant virus: dissimilar biological behaviour regarding its parental genotypes.Semantic proximity Read ↗

Phenotyping hepatitis B virus variants: from transfection towards a small animal in vivo infection model.Semantic proximity Read ↗

Recombinant virus assay: a rapid, phenotypic assay for assessment of drug susceptibility of human immunodeficiency virus type 1 isolates.Semantic proximity Read ↗

In vitro drug susceptibility analysis of hepatitis B virus clinical quasispecies populations.Semantic proximity Read ↗

Hepatitis B virus mutations potentially conferring adefovir/tenofovir resistance in treatment-naive patients.Semantic proximity Read ↗

The rtA194T polymerase mutation impacts viral replication and susceptibility to tenofovir in hepatitis B e antigen-positive and hepatitis B e antigen-negative hepatitis B virus strains.Semantic proximity Read ↗

Novel recombinant phenotypic assay for clonal analysis of reverse transcriptase mutations conferring drug resistance to HIV-1 variants.Semantic proximity Read ↗

In vitro characterization of six hepatitis B virus genotypes from clinical isolates using transfecting linear HBV genomes.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.