Article
A large-scale in vivo analysis reveals that TALENs are significantly more mutagenic than ZFNs generated using context-dependent assembly.
Nucleic acids research - 1 Feb 2013
Chen Shijia, Oikonomou Grigorios, Chiu Cindy N, Niles Brett J, Liu Justin, Lee Daniel A, Antoshechkin Igor, Prober David A
Abstract excerpt
Zinc-finger nucleases (ZFNs) and TAL effector nucleases (TALENs) have been shown to induce targeted mutations, but they have not been extensively tested in any animal model. Here, we describe a large-scale comparison of ZFN and TALEN mutagenicity in zebrafish. Using deep sequencing, we found that TALENs are significantly more likely to be mutagenic and induce an average of 10-fold more mutations than ZFNs. We...
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