Article
Highly efficient generation of heritable zebrafish gene mutations using homo- and heterodimeric TALENs.
Nucleic acids research - 1 Sept 2012
Cade Lindsay, Reyon Deepak, Hwang Woong Y, Tsai Shengdar Q, Patel Samir, Khayter Cyd, Joung J Keith, Sander Jeffry D, Peterson Randall T, Yeh Jing-Ruey Joanna
Abstract excerpt
Transcription activator-like effector nucleases (TALENs) are powerful new research tools that enable targeted gene disruption in a wide variety of model organisms. Recent work has shown that TALENs can induce mutations in endogenous zebrafish genes, but to date only four genes have been altered, and larger-scale tests of the success rate, mutation efficiencies and germline transmission rates have not been...
Topics
- Alleles
- Animals
- Base Sequence
- DNA-Binding Proteins
- Deoxyribonucleases, Type II Site-Specific
- Dimerization
- Gene Knockout Techniques
- Molecular Sequence Data
- Mutagenesis
- Mutation
- Trans-Activators
- Zebrafish