Article
High-throughput genome editing and phenotyping facilitated by high resolution melting curve analysis.
PloS one - 1 Jan 2014
Thomas Holly R, Percival Stefanie M, Yoder Bradley K, Parant John M
Abstract excerpt
With the goal to generate and characterize the phenotypes of null alleles in all genes within an organism and the recent advances in custom nucleases, genome editing limitations have moved from mutation generation to mutation detection. We previously demonstrated that High Resolution Melting (HRM) analysis is a rapid and efficient means of genotyping known zebrafish mutants. Here we establish optimized conditions...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.