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Article

Evaluation and application of modularly assembled zinc-finger nucleases in zebrafish.

Development (Cambridge, England) - 1 Oct 2011

Zhu Cong, Smith Tom, McNulty Joseph, Rayla Amy L, Lakshmanan Abirami, Siekmann Arndt F, Buffardi Matthew, Meng Xiangdong, Shin Jimann, Padmanabhan Arun, Cifuentes Daniel, Giraldez Antonio J, Look A Thomas, Epstein Jonathan A, Lawson Nathan D, Wolfe Scot A

Abstract excerpt

Zinc-finger nucleases (ZFNs) allow targeted gene inactivation in a wide range of model organisms. However, construction of target-specific ZFNs is technically challenging. Here, we evaluate a straightforward modular assembly-based approach for ZFN construction and gene inactivation in zebrafish. From an archive of 27 different zinc-finger modules, we assembled more than 70 different zinc-finger cassettes and...

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Share to Animals, Genetically Modified
Share to Deoxyribonucleases, Type II Site-Specific

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to GATA2 Transcription Factor

Share to Gene Targeting

Share to Neovascularization, Physiologic

Share to Zebrafish

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Source

Open in PubMed Open full text

Identifiers

PMID: 21937602
DOI: 10.1242/dev.066779
PMCID: PMC3177320

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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