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Automating gene library synthesis by structure-based combinatorial protein engineering: examples from plant sesquiterpene synthases.

Methods in enzymology - 1 Jan 2012

Dokarry Melissa, Laurendon Caroline, O'Maille Paul E

Abstract excerpt

Structure-based combinatorial protein engineering (SCOPE) is a homology-independent recombination method to create multiple crossover gene libraries by assembling defined combinations of structural elements ranging from single mutations to domains of protein structure. SCOPE was originally inspired by DNA shuffling, which mimics recombination during meiosis, where mutations from parental genes are "shuffled" to...

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PMID: 22999168
DOI: 10.1016/B978-0-12-394290-6.00002-1

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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