Article
Rapid pathway prototyping and engineering using in vitro and in vivo synthetic genome SCRaMbLE-in methods.
Nature communications - 22 May 2018
Liu Wei, Luo Zhouqing, Wang Yun, Pham Nhan T, Tuck Laura, Pérez-Pi Irene, Liu Longying, Shen Yue, French Chris, Auer Manfred, Marles-Wright Jon, Dai Junbiao, Cai Yizhi
Abstract excerpt
Exogenous pathway optimization and chassis engineering are two crucial methods for heterologous pathway expression. The two methods are normally carried out step-wise and in a trial-and-error manner. Here we report a recombinase-based combinatorial method (termed "SCRaMbLE-in") to tackle both challenges simultaneously. SCRaMbLE-in includes an in vitro recombinase toolkit to rapidly prototype and diversify gene...
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