Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Chemical and biochemical strategies for the randomization of protein encoding DNA sequences: library construction methods for directed evolution.

Nucleic acids research - 1 Jan 2004

Neylon Cameron

Abstract excerpt

Directed molecular evolution and combinatorial methodologies are playing an increasingly important role in the field of protein engineering. The general approach of generating a library of partially randomized genes, expressing the gene library to generate the proteins the library encodes and then screening the proteins for improved or modified characteristics has successfully been applied in the areas of...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Directed Molecular Evolution
Share to DNA
Share to Gene Library

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Oligonucleotides

Share to Protein Engineering

Share to Proteins

Share to Random Allocation

Share to Recombination, Genetic

Source

Open in PubMed Open full text

Identifiers

PMID: 14990750
DOI: 10.1093/nar/gkh315
PMCID: PMC390300

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Chemical and biochemical strategies for the randomization of protein encoding DNA sequences: library construction methods for directed evolution.PMID 14990750Read ↗

Steering directed protein evolution: strategies to manage combinatorial complexity of mutant libraries.Semantic proximity Read ↗

A Brief Guide to the High-Throughput Expression of Directed Evolution Libraries.Semantic proximity Read ↗

[Progress in the construction and screening of random mutation library].Semantic proximity Read ↗

Construction and analysis of randomized protein-encoding libraries using error-prone PCR.Semantic proximity Read ↗

Directed evolution combined with synthetic biology strategies expedite semi-rational engineering of genes and genomes.Semantic proximity Read ↗

Strategies and computational tools for improving randomized protein libraries.Semantic proximity Read ↗

The diversity challenge in directed protein evolution.Semantic proximity Read ↗

Accelerated protein engineering for chemical biotechnology via homologous recombination.Semantic proximity Read ↗

The evolving art of creating genetic diversity: From directed evolution to synthetic biology.Semantic proximity Read ↗

Combinatorial library approaches for improving soluble protein expression in Escherichia coliSemantic proximity Read ↗

Advances in directed protein evolution by recursive genetic recombination: applications to therapeutic proteins.Semantic proximity Read ↗

Synthetic gene libraries: in search of the optimal diversity.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.