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Article

Construction and engineering of large biochemical pathways via DNA assembler.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2013

Shao Zengyi, Zhao Huimin

Abstract excerpt

DNA assembler enables rapid construction and engineering of biochemical pathways in a one-step fashion by exploitation of the in vivo homologous recombination mechanism in Saccharomyces cerevisiae. It has many applications in pathway engineering, metabolic engineering, combinatorial biology, and synthetic biology. Here we use two examples including the zeaxanthin biosynthetic pathway and the aureothin...

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Identifiers

PMID: 23996442
DOI: 10.1007/978-1-62703-625-2_9
PMCID: PMC4321867

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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