Article
In vitro DNA SCRaMbLE.
Nature communications - 22 May 2018
Wu Yi, Zhu Rui-Ying, Mitchell Leslie A, Ma Lu, Liu Rui, Zhao Meng, Jia Bin, Xu Hui, Li Yun-Xiang, Yang Zu-Ming, Ma Yuan, Li Xia, Liu Hong, Liu Duo, Xiao Wen-Hai, Zhou Xiao, Li Bing-Zhi, Yuan Ying-Jin, Boeke Jef D
Abstract excerpt
The power of synthetic biology has enabled the expression of heterologous pathways in cells, as well as genome-scale synthesis projects. The complexity of biological networks makes rational de novo design a grand challenge. Introducing features that confer genetic flexibility is a powerful strategy for downstream engineering. Here we develop an in vitro method of DNA library construction based on structural...
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