International Pig-a gene mutation assay trial: evaluation of transferability across 14 laboratories.

doi:10.1002/em.20672

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International Pig-a gene mutation assay trial: evaluation of transferability across 14 laboratories.

Environmental and molecular mutagenesis - 1 Dec 2011

Dertinger Stephen D, Phonethepswath Souk, Weller Pamela, Nicolette John, Murray Joel, Sonders Paul, Vohr Hans-Werner, Shi Jing, Krsmanovic Ljubica, Gleason Carol, Custer Laura, Henwood Andrew, Sweder Kevin, Stankowski Leon F, Roberts Daniel J, Giddings Amanda, Kenny Julia, Lynch Anthony M, Defrain Céline, Nesslany Fabrice, van der Leede Bas-jan M, Van Doninck Terry, Schuermans Ann, Tanaka Kentaro, Hiwata Yoshie, Tajima Osamu, Wilde Eleanor, Elhajouji Azeddine, Gunther William C, Thiffeault Catherine J, Shutsky Thomas J, Fiedler Ronald D, Kimoto Takafumi, Bhalli Javed A, Heflich Robert H, MacGregor James T

Abstract excerpt

A collaborative international trial was conducted to evaluate the reproducibility and transferability of an in vivo mutation assay based on the enumeration of CD59-negative rat erythrocytes, a phenotype that is indicative of Pig-a gene mutation. Fourteen laboratories participated in this study, where anti-CD59-PE, SYTO 13 dye, and flow cytometry were used to determine the frequency of CD59-negative erythrocytes...

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PMID: 21910140
DOI: 10.1002/em.20672

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