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In Vivo Pig-a gene mutation assay: Guidance for 3Rs-friendly implementation.

Environmental and molecular mutagenesis - 1 Dec 2016

Raschke Marian, Igl Bernd-W, Kenny Julia, Collins Joanne, Dertinger Stephen D, Labash Carson, Bhalli Javed A, Tebbe Cameron C M, McNeil Kylie M, Sutter Andreas

Abstract excerpt

The rodent Pig-a assay is an in vivo method for the detection of gene mutation, where lack of glycosylphosphatidylinositol-anchored proteins on the surface of circulating red blood cells (RBCs) serves as a reporter for Pig-a gene mutation. In the case of rats, the frequency of mutant phenotype RBCs is measured via fluorescent anti-CD59 antibodies and flow cytometry. The Pig-a assay meets the growing expectations...

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PMID: 27770464
DOI: 10.1002/em.22060

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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