Article
[Optimization ofPig-aGene Mutation in Rats and Assessment of Time-dependent and Dose-response Relationship of N-ethyl-N-nitrosourea].
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition - 1 Jan 2017
Ma Si-Jia, Huo Jiao, Chen Jin-Yao, Chen Qi-Yan, Zhang Li-Shi
Abstract excerpt
OBJECTIVES: To optimize the method of Pig-a mutation assay, and to explore the time-dependent and dose-response relationship of N-ethyl-N-nitrosourea (ENU). METHODS: Thirty rats were randomly assigned to 5 groups: treated with PBS (control group)or different doses of ENU (10, 20, 40 and 80 mg/kg) for 3 d by oral gavage. Blood samples were collected at 0 d, 15 d, 30 d, 45 d, 60 d, 75 d and 90 d. After enrichment,...
Topics
- Animals
- Dose-Response Relationship, Drug
- Erythrocytes
- Ethylnitrosourea
- Membrane Proteins
- Mutation
- Rats
- Reticulocytes
- Time Factors