Article
Metallothioneins with interdomain hinges expanded by insertion mutagenesis.
Protein engineering - 1 Jan 1990
Rhee I K, Lee K S, Huang P C
Abstract excerpt
Specific peptides of varying lengths were inserted between the two metal cluster domains of metallothionein (MT), which normally are spanned by only three amino acids, Lys-Lys-Ser. These interdomain expansions were made to test if such structural alterations would affect MT function. These constr...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Cell Line
- Cloning, Molecular
- Codon
- Cricetinae
- DNA
- Drug Tolerance
- Escherichia coli
- Exons
- Metallothionein
- Metals
- Molecular Sequence Data
- Mutation
- Plasmids
- RNA, Messenger
- Repetitive Sequences, Nucleic Acid