Article
Selection of mouse cells with amplified metallothionein genes retaining their glucocorticoid inducibility.
FEBS letters - 21 Sept 1992
Thibodeau J, De Souza C, Smorawinska M, Thirion J P
Abstract excerpt
Two new mouse cell mutants, resistant to either 80 or 100 mM CdCl2, were isolated to study the regulation of transcription by the glucocorticoid hormones. Their metallothionein mt-1% and mt-2+ genes were amplified coordinately to a maximum of 30 copies per cell. By Southern blot analysis, no gross rearrangement was detectable near the mt+ loci. Contrary to other mutants previously isolated, the...
Topics
- Animals
- Autoradiography
- Blotting, Northern
- Blotting, Southern
- Cadmium
- Cells, Cultured
- DNA
- Dexamethasone
- Electrophoresis, Polyacrylamide Gel
- Gene Amplification
- Gene Expression