Article
Expression of a Neurospora crassa metallothionein and its variants in Escherichia coli.
Applied and environmental microbiology - 1 Sept 1990
Romeyer F M, Jacobs F A, Brousseau R
Abstract excerpt
The Neurospora crassa metallothionein (NC) synthesis gene was cloned and expressed in Escherichia coli in two different expression vectors (pING2 and pUA7), both under the regulation of the Salmonella typhimurium arabinose operon. Upon induction with arabinose, the pING2-NC vector expressed as inclusion body-localized AraB'::NC fusion protein of 21 kilodaltons. The pUA7-NC vector expressed a 5.3-kilodalton...
Topics
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- DNA, Fungal
- Escherichia coli
- Gene Expression
- Genes, Fungal
- Metallothionein
- Metals
- Molecular Sequence Data
- Mutation