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Article

Use of fortuitous in vitro mutations in a synthetic Cu-Zn superoxide dismutase gene to illuminate protein structure-function relationships.

Protein engineering - 1 Nov 1998

Kanan J H, Hubbard S J, Minter S J, Oliver S G

Abstract excerpt

A completely synthetic bovine copper-zinc superoxide dismutase gene (Cu-ZnSOD), designed using the most favoured codons for expression in yeast, was constructed. Fortuitous mutations introduced while cloning the synthetic gene permitted the additional construction of four altered-polypeptide prod...

Topics

Amino Acid Sequence
Animals
Base Sequence
Cattle
Cloning, Molecular
DNA, Recombinant
Molecular Sequence Data
Mutation
Recombinant Proteins
Superoxide Dismutase

Source

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 9876930
DOI: 10.1093/protein/11.11.1083

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-9876930-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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