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Article

Use of fortuitous in vitro mutations in a synthetic Cu-Zn superoxide dismutase gene to illuminate protein structure-function relationships.

Protein engineering - 1 Nov 1998

Kanan J H, Hubbard S J, Minter S J, Oliver S G

Abstract excerpt

A completely synthetic bovine copper-zinc superoxide dismutase gene (Cu-ZnSOD), designed using the most favoured codons for expression in yeast, was constructed. Fortuitous mutations introduced while cloning the synthetic gene permitted the additional construction of four altered-polypeptide products representing two single (Pro121-->Leu and Gly128-->Asp), one double (Pro100-->Leu, Arg113-->Lys) and one triple...

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Identifiers

PMID: 9876930
DOI: 10.1093/protein/11.11.1083

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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