Article
Mutation of invariant cysteines of mammalian metallothionein alters metal binding capacity, cadmium resistance, and 113Cd NMR spectrum.
The Journal of biological chemistry - 25 Dec 1991
Cismowski M J, Narula S S, Armitage I M, Chernaik M L, Huang P C
Abstract excerpt
Using a yeast expression vector system, we have expressed both wild type and six mutated Chinese hamster metallothionein coding sequences in a metal-sensitive yeast strain in which the endogenous metallothionein gene has been deleted. The mutant proteins have single or double cysteine to tyrosine replacements (C13Y, C50Y, and C13,50Y), single cysteine to serine replacements (C13S and C50S), or a single cysteine...
Topics
- Animals
- Base Sequence
- Cadmium
- Cloning, Molecular
- Cricetinae
- Cricetulus
- Cysteine
- Electrophoresis, Polyacrylamide Gel
- Isotopes
- Magnetic Resonance Spectroscopy
- Metallothionein