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Article

Pipeline for large-scale microdroplet bisulfite PCR-based sequencing allows the tracking of hepitype evolution in tumors.

PloS one - 1 Jan 2011

Herrmann Alexander, Haake Andrea, Ammerpohl Ole, Martin-Guerrero Idoia, Szafranski Karol, Stemshorn Kathryn, Nothnagel Michael, Kotsopoulos Steve K, Richter Julia, Warner Jason, Olson Jeff, Link Darren R, Schreiber Stefan, Krawczak Michael, Platzer Matthias, Nürnberg Peter, Siebert Reiner, Hampe Jochen

Abstract excerpt

Cytosine methylation provides an epigenetic level of cellular plasticity that is important for development, differentiation and cancerogenesis. We adopted microdroplet PCR to bisulfite treated target DNA in combination with second generation sequencing to simultaneously assess DNA sequence and methylation. We show measurement of methylation status in a wide range of target sequences (total 34 kb) with an average...

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Share to Colonic Neoplasms
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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Share to Genome, Human

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Identifiers

PMID: 21750708
DOI: 10.1371/journal.pone.0021332
PMCID: PMC3130030

Genes

FGF6
SNRPN

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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