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Article

A streamlined method for analysing genome-wide DNA methylation patterns from low amounts of FFPE DNA

31 Aug 2017

Abstract excerpt

BACKGROUND: Formalin fixed paraffin embedded (FFPE) tumor samples are a major source of DNA from patients in cancer research. However, FFPE is a challenging material to work with due to macromolecular fragmentation and nucleic acid crosslinking. FFPE tissue particularly possesses challenges for methylation analysis and for preparing sequencing-based libraries relying on bisulfite conversion. Successful bisulfite...

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PMID: 28859641
DOI: 10.1186/s12920-017-0290-1

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